The Protein Maker: an automated system for high-throughput parallel purification
نویسندگان
چکیده
The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications.
منابع مشابه
Application of the Protein Maker as a platform purification system for therapeutic antibody research and development
Within the research and development environment, higher throughput, parallelized protein purification is required for numerous activities, from small scale purification of monoclonal antibodies (mAbs) and antibody fragments for in vitro and in vivo assays to process development and optimization for manufacturing. Here, we describe specific applications and associated workflows of the Protein Ma...
متن کاملDevelopment of an automated mid-scale parallel protein purification system for antibody purification and affinity chromatography.
Protein purification is often a bottleneck during protein generation for large molecule drug discovery. Therapeutic antibody campaigns typically require the purification of hundreds of monoclonal antibodies (mAbs) during the hybridoma process and lead optimization. With the increase in high-throughput cloning, faster DNA sequencing, and the use of parallel protein expression systems, a need for...
متن کاملRetrieval–travel-time model for free-fall-flow-rack automated storage and retrieval system
Automated storage and retrieval systems (AS/RSs) are material handling systems that are frequently used in manufacturing and distribution centers. The modelling of the retrieval–travel time of an AS/RS (expected product delivery time) is practically important, because it allows us to evaluate and improve the system throughput. The free-fall-flow-rack AS/RS has emerged as a new technology for dr...
متن کاملAn automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...
متن کاملAutomating FFPE Samples from Extraction to NGS Library Preparation
Building on our established automation and applications expertise across the genomics workflow, we describe an automated workflow partnering with Covaris®, Inc. to prepare NGS libraries from FFPE tissues samples (Figure 1). In this application note, we demonstrate an automated, high-throughput workflow that encompasses DNA extraction, purification, sample quality assessment, and subsequent libr...
متن کامل